Allele/Variant

Awat2tm1.2Golc

Species
Mus musculus
Symbol
Awat2tm1.2Golc
Category
allele
Allele of gene
Awat2
Transgenic Constructs
None
Synonyms
None
Description
A loxP site was inserted 371 bp upstream of exon 3, and an FRT site, loxP site, neomycin resistance gene, another FRT site and a third loxP site were inserted 351 bp downstream of exon 5. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination deleted exons 3-5. Excision of these exons eliminates the part of the enzyme containing the catalytic residues 107HPGH110 encoded in exon 3 and introduces a stop codon following exons 6 and 7. RT-qPCR confirmed absence of transcript and immunofluorescence of skin and tarsal plate shows absence of protein.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

Phenotypes

Phenotype
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    Disease Associations

    Association
    Disease Qualifier
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