Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Tg(Mchr1-cre/ERT2)3Nbe
- Species
- Mus musculus
- Symbol
- Tg(Mchr1-cre/ERT2)3Nbe
- Category
- allele
- Allele of gene
- None
- Transgenic Constructs
- Synonyms
- Mchr1-CreER
- Description
- A cre/ERT2 expression sequence was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting Mchr1-cre/ERT2 BAC transgene was purified for subsequent male pronuclear injection to establish transgenic founder animals. Two lines 2 and 3 were generated with similar expression patterns. .
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- None
- Knock-down Targets
- None
- Regulatory Regions