Allele/Variant

Tg(Sprr2f-cre)2Dcas

Species
Mus musculus
Symbol
Tg(Sprr2f-cre)2Dcas
Category
allele
Allele of gene
None
Transgenic Constructs
Synonyms
  • BAC-Sprr2f-Cre
Description
Using a 189 kb BAC clone (RP23-39I4) containing the entire mouse Sprr2f gene, the cre and neo cassette was inserted into the ATG start codon in exon 2 of the mouse Sprr2f gene via bacterial recombineering method. The neo selection cassette was then excised by transient flippase expression, and the resulting engineered BAC was injected into mouse oocytes for the generation of transgenic founder lines. Five founders had cre insertion, the line 2 was assigned to this transgene in absence of specific line information to distinguish it from Tg(Sprr2f-cre)1Dcas generated by the same creator which showed ectopic cre activity in brain and kidney. This transgene shows specific cre activity only in the uterus and no ectopic expression was observed.
Additional Information
Literature

Transgenic Constructs

This allele contains 1 transgenic construct
Symbol
Expressed Components
None
Knock-down Targets
None
Regulatory Regions
All alleles with this construct

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations