Allele/Variant

Rangrftm1.1Tke

Species
Mus musculus
Symbol
Rangrftm1.1Tke
Category
allele
Allele of gene
Rangrf
Transgenic Constructs
None
Synonyms
  • Mog1-
Description
Three premature stop codons, V31* (GTG to TAG), P32* (CCA to TGA), and D33* (GAC to TAA) were introduced in exon 2, which is expected to result in nonsense-mediated decay of mRNA, along with a loxP-flanked neomycin resistance gene following the mutated exon 2. The selection cassette was removed via cre-mediated recombination. This knock-in strategy was used to knock-out the gene without affecting the partially overlapping Slc25a35 gene (transcribed in opposite direction). RT-qPCR analysis shows 90% reduction in cardiac mRNA of homozygotes. Western blot analysis shows nearly complete loss of expression in homozygous heart.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations