Allele/Variant

Esm1tm1.1Rha

Species
Mus musculus
Symbol
Esm1tm1.1Rha
Category
allele
Allele of gene
Esm1
Transgenic Constructs
None
Synonyms
  • Esm1KO
Description
LoxP recognition sites were inserted around the second exon of the murine Esm1 gene via homologous recombination in the ES cells. A murine cDNA fragment containing the coding sequence from exons 2 and 3 followed by 2 copies of the bovine growth hormone polyadenylation signal sequence was inserted in frame, and downstream of the loxP-flanked area, the targeting construct also contained a beta-galactosidase (lacZ) reporter cassette, which carried an artificial splice acceptor and an internal ribosomal entry site (IRES) at the 5’-end and the SV40 polyadenylation signal sequence at the 3’-end. The targeting construct also contained a neomycin resistance cassette surrounded by Frt sites allowing Flp recombinase-mediated removal. Through interbreeding with PGK-Cre deleter mice, exon 2 of the Esm1 gene was globally deleted. No beta-galactosidase reporter activity was detectable in heterozygous or homozygous mutants, and it was subsequently confirmed that Esm1-lacZ fusion transcripts were not detectable by qPCR.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

No data available

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    Disease Associations

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