Allele/Variant

Rhoqtm1b(EUCOMM)Hmgu

Species
Mus musculus
Symbol
Rhoqtm1b(EUCOMM)Hmgu
Category
allele
Allele of gene
Rhoq
Transgenic Constructs
None
Synonyms
  • TC10tm1b
Description
The L1L2_Bact_P cassette was inserted at position 87271472 of Chromosome 17 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 87271906. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked exon 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Immunoblot analysis confirmed the absence of protein expression in brain, liver, and lung homogenates isolated from adult homozygous mutant mice.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations