CRISPR/cas9 mediated recombination in zygotes from a cross of females homozygous for Atg16l1tm1c(EUCOMM)Wtsi and males homozygous for Atg16l1tm1c(EUCOMM)Wtsi and carrying Tg(Vil1-cre)997Gum> created a 2 nucleotide insertion of AT after the third codon resulting in a frameshift and premature stop at amino acid position 30.