Allele/Variant

Limch1em1Coop

Species
Mus musculus
Symbol
Limch1em1Coop
Category
allele
Allele of gene
Limch1
Transgenic Constructs
None
Synonyms
  • Limch1 6exKO
Description
In mouse, the skeletal muscle-specific isoform contains 6 additional exons included after birth and is the predominant isoform in adult skeletal muscle. CRISPR/Cas9 genome editing technology was used to delete exons 9-14 encoding the skeletal muscle-specific isoform, thereby forcing the constitutive expression of the ubiquitous isoform that normally predominates in fetal skeletal muscle. RT-PCR analysis demonstrated the absence of the 6 alternatively spliced exons and the correct splicing of the flanking constitutive exons 8 and 15 that are included in RNA from knockout skeletal muscle tissues. Western blotting confirmed that knockout mice lack protein expression of the skeletal muscle-specific isoform and exclusively express the fetal predominant isoform in adult skeletal (quadriceps, EDL, and soleus) muscle.
Additional Information
Literature

Transgenic Constructs

No data available

Genomic Variant Information

No data available

Variant Molecular Consequences

Phenotypes

Disease Associations