Enables diphthine-ammonia ligase activity. Involved in protein histidyl modification to diphthamide. Located in cytoplasm. Orthologous to human DPH6 (diphthamine biosynthesis 6).
SGD Description
Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene