Gene

cps-6

Species
Caenorhabditis elegans
Symbol
cps-6
Name
CED-3 Protease Suppressor 6
Synonyms
  • C41D11.8
  • CELE_C41D11.8
Biotype
protein coding gene
Automated Description
Enables endonuclease activity; protein homodimerization activity; and sequence-specific DNA binding activity. Involved in RNA catabolic process and apoptotic DNA fragmentation. Located in mitochondrion. Orthologous to human ENDOG (endonuclease G).
WB Description
cps-6 encodes an ortholog of human mitochondrial endonuclease G (EndoG) that promotes apoptosis, and is required to degrade nicked (TUNEL-positive) DNA in apoptotic cells; transgenic CPS-6 is localized to mitochondria, but transgenic CPS-6 lacking a mitochondrial localization sequence is found in nuclei, and nuclei may be the in vivo target of CPS-6 after its activation by CED-3; CPS-6 has magnesium-dependent nuclease activity in vitro and can degrade both single- and double-stranded DNA, as well as single-stranded RNA; CPS-6 also preferentially binds G-tract DNA in vitro; cps-6(sm116) and cps-6(RNAi) animals have delayed CED-3-induced apoptosis, and cps-6(sm116) suppresses a constitutively active ced-3 transgene; cps-6(sm116) can be transgenically rescued by mouse EndoG; CPS-6 binds WAH-1 (an apoptosis-inducing factor ortholog) in vitro, WAH-1 binding enhances CPS-6's endonuclease activity, and constitutive transgenic coexpression of cps-6 with wah-1 induces cell death not seen with constitutive expression of either cps-6 or wah-1 alone; CPS-6 binds CRN-1 (a flap endonuclease ortholog) in vitro, and may form a large complex in vivo with CRN-1, CRN-4, CRN-5, CYP-13, and WAH-1; CRN-1 enhances CPS-6's endonuclease activity in vitro, CPS-6 enhances CRN-1's gap-dependent endonuclease and 5'-3' exonuclease activities, and cps-6 is required for excess cell deaths induced by a crn-1 transgene.
Cross References
Additional Information
Literature

Orthology

Gene tree
PANTHER:PTHR13966
Links to orthology data in JBrowse by filter level: Stringent,  Moderate,  No filter,  Best and Best Reverse

Paralogy

Function - GO Annotations

Pathways

No data available

Phenotypes

Primary Sources
Other Sources
None

Disease Associations

Cases where the expected disease association was NOT found
Cell color indicative of annotation volume

Transgenic Alleles

Models

Sequence Feature Viewer

Genome location
Assembly version
WBcel235
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4.4466M4.4468M4.4470M4.4472M4.4474M4.4476M4.4478M4.4480M4.4482M4.4484M

Sequence Details

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Expression

Primary Sources
Other Sources
Cell color indicative of annotation volume; red slash indicates species lacks structure or developmental stage.

Molecular Interactions

Genetic Interactions