Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase binding activity. Involved in programmed cell death involved in cell development; protein ubiquitination; and ubiquitin-dependent protein catabolic process. Predicted to be located in chromosome and cytoplasm. Predicted to be active in nucleus. Is expressed in several structures, including P1; P10; linker cell; ventral ganglion; and ventral nerve cord. Orthologous to several human genes including UBE2D2 (ubiquitin conjugating enzyme E2 D2) and UBE2D3 (ubiquitin conjugating enzyme E2 D3).
WB Description
let-70 encodes a class I E2 ubiquitin conjugating enzyme; let-70 was identified in screens for essential genes and phenotypic analyses indicate that loss of let-70 activity results in embryonic and larval lethality with defects in sarcomere assembly; purified LET-70 can stimulate the self-ubiquitylation activities of CHN-1 and UFD-2, E4 ubiquitin conjugation factors and, in addition, can stimulate the CHN-1- and UFD-2-dependent multiubiquitylation of UNC-45, a myosin-directed chaperone; in this manner, LET-70 may regulate UNC-45 degradation and myosin assembly; RNA blot analyses and a let-70-lacZ reporter fusion reveal that let-70 is abundantly expressed at all stages of development, including the dauer larval stage; strong staining is observed in most somatic tissues until adult stages, when expression generally becomes restricted to the nervous system.