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Summary: DDX3X is frequently mutated in the WNT and SHH subtypes of medulloblastom-the commonest malignant childhood brain tumor. But whether DDX3X functions as a medulloblastoma oncogene or tumor suppressor gene is not known. Here we show that Ddx3x regulates hindbrain patterning and development by controlling Hox gene expression and cell stress signaling. In mice predisposed to Wnt or Shh-medulloblastoma Ddx3x sensed oncogenic stress and suppressed tumor formation. WNT and SHH-medulloblastomas normally arise only in the lower and upper rhombic lips respectively. Deletion of Ddx3x relived this lineage restriction enabling both medulloblastoma subtypes to arise in either germinal zone. Thus DDX3X is a medulloblastoma tumor suppressor that regulates hindbrain development and restricts the competence of cell lineages to form medulloblastoma subtypes. RNA-sequencing profiles of developmental time points in the cerebullum of WT and DDX3X perturbed Blbp positive cells and in tumors formed from aberrant Wnt or Shh signalling. BCP and BPtcH DO NOT have DDX3X perturbations. 3 to 5 replicates per time point per condition.

Symbol: DDX3X suppresses neural lineage susceptibility to medulloblastoma [RNA-seq]

Name: DDX3X suppresses neural lineage susceptibility to medulloblastoma [RNA-seq]

Identification of DDX3X-dependent translation targets in the E11.5 mouse cortex

(Mus musculus)

HTP Dataset Index

High-Throughput (HTP) Dataset Index metadata provided by MGI

ID: ArrayExpress:GSE203078

Tags: sex, WT vs. mutant, genotype

Summary: To identify DDX3X-dependent translation targets in the developing mouse cortex, we used E11.5 cortices from both sexes of wildtype and conditional Ddx3x knockout mice (driven by Emx1-Cre). We performed RNAseq and Riboseq on these samples in parallel. Comparative gene expression profiling analysis of RNA-seq and Ribo-seq data for wildtype and Ddx3x conditional knockout mouse E11.5 cortex. The following are biological replicates: Female controls: F2, F3; Male controls: M1, M2, M3; cKO females: F1, F2, F3; cKO mals: M1, M2, M3

Symbol: Identification of DDX3X-dependent translation targets in the E11.5 mouse cortex

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