Version: 8.0.0Date: Tue Jan 28 2025
HTP Dataset Index
HTP Dataset Index
High-Throughput (HTP) Dataset Index metadata provided by MGI
ID: ArrayExpress:E-GEOD-37514, GEO:GSE37514
Tags: WT vs. mutant, genotype
Summary: We identified secreted frizzled-related protein-5 (Sfrp5) as a transcript that is upregulated during adipocyte differentiation and that is increased in white adipose tissue (WAT) of obese mice, compared to lean mice. To investigate the function of sFRP5 in adipose tissue biology, we studied sFRP5Q27stop mice, in which ENU mutagenesis was used to create a premature stop codon at Gln27, thereby creating a likely null allele. Male wild-type or Sfrp5 KO (Q27Stop) mice were fed a high-fat diet from the ages of four weeks to twelve weeks. At twelve weeks of age, mice were euthanized. Total RNA was then isolated from gonadal WAT and RNA was analyzed by Affymetrix microarrays. Seven wild-type and eight Sfrp5 KO mice were used.
Symbol: Effect of ablation of Sfrp5 on gene expression in gonadal white adipose tissue
Name: Effect of ablation of Sfrp5 on gene expression in gonadal white adipose tissue
Secreted Frizzled-Related Protein 5 is Downregulated in Obesity and Promotes ß-Cell Proliferation [30 days]
(Rattus norvegicus)HTP Dataset Index
High-Throughput (HTP) Dataset Index metadata provided by RGD
ID: GEO:GSE44046
Tags: unclassified
Summary: Obesity is associated with an increase in ß-cell mass in response tothe rising demand for insulin. ß-cell plasticity is essential to maintaining glucose homeostasis, however,the cellular and molecular mechanisms by which ß-cell mass is regulated remain poorly understood.Recently, we described the existence of a crosstalk between the peripancreatic adipose tissue and ß-cells as a novel mechanism that participates in the regulation of ß-cell plasticity. Here, we identify the secreted frizzled-related protein (Sfrp) 5 as down-regulated in the pancreatic islets of obese rats as well as in the pancreatic islets of human obese patients. Our results demonstrate that the silencing of Sfrp5 induces an increase in ß-cell proliferation, which we correlate with the activation of Wnt signaling and of the MAPK and PI3 kinase pathways. Together, these findings expand our understanding of the mechanisms underlying ß-cell proliferation under conditions of obesity. Furthermore, this study opens new insights into the specific targeting of Sfrp5 as a novel therapeutic strategy for balancing ß-cell mass.
Secreted Frizzled-Related Protein 5 is Downregulated in Obesity and Promotes ß-Cell Proliferation [10 days]
(Rattus norvegicus)HTP Dataset Index
High-Throughput (HTP) Dataset Index metadata provided by RGD
ID: GEO:GSE44045
Tags: unclassified
Summary: Obesity is associated with an increase in ß-cell mass in response tothe rising demand for insulin. ß-cell plasticity is essential to maintaining glucose homeostasis, however,the cellular and molecular mechanisms by which ß-cell mass is regulated remain poorly understood.Recently, we described the existence of a crosstalk between the peripancreatic adipose tissue and ß-cells as a novel mechanism that participates in the regulation of ß-cell plasticity. Here, we identify the secreted frizzled-related protein (Sfrp) 5 as down-regulated in the pancreatic islets of obese rats as well as in the pancreatic islets of human obese patients. Our results demonstrate that the silencing of Sfrp5 induces an increase in ß-cell proliferation, which we correlate with the activation of Wnt signaling and of the MAPK and PI3 kinase pathways. Together, these findings expand our understanding of the mechanisms underlying ß-cell proliferation under conditions of obesity. Furthermore, this study opens new insights into the specific targeting of Sfrp5 as a novel therapeutic strategy for balancing ß-cell mass.
Agilent microarray analysis between a Dullard-heterozygous and a Dullard-homozygous E7.5 embryo
(Mus musculus)HTP Dataset Index
High-Throughput (HTP) Dataset Index metadata provided by MGI
ID: ArrayExpress:E-GEOD-39224, GEO:GSE39224
Tags: WT vs. mutant, genotype
Summary: To explore the loss of Dullurd function in mouse embryo development, we have performed the Agilent microarray analysis between a Dullard-heterozygous and a Dullard-homozygous E7.5 embryo. Our findings show that Dullard does not act in concert with BMP4 and Smad1/5/8, whereas loss of Dullard is associated with a reduced level of WNT/beta-catenin signaling activity, accompanied by elevated expression of Wnt3 and WNT antagonists including Dkk1, Sfrp1 and Sfrp5 in mouse germ cell formation. E7.5 mouse embryos were collected. One Dullard-heterozygous embryo and one Dullard-homozygous embryo were used for the analysis.
Mice model for 15q11-13 duplication syndrome show late onset obesity and altered lipid metabolism
(Mus musculus)HTP Dataset Index
High-Throughput (HTP) Dataset Index metadata provided by MGI
ID: ArrayExpress:E-GEOD-58191, GEO:GSE58191
Tags: WT vs. mutant, genotype
Summary: To investigate the gene expression change in the white adipose tissue (WAT) of patDp/+ mice compared with wild type mice, the whole genome microarray profiling was performed. Total RNA from WAT of young adult stage mice (9-10 weeks old) was extracted and subject to microarray analysis. Expression change of sfrp5 gene was further validated by real-time PCR, suggesting this gene might contribute to the obesity in patDp/+ mice. We measured the gene expression change in WAT of young adult stage mice. Three patDp/+ mice and three wild type mice were used in the experiment.