Il1b^tm1Dch

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Species

Mus musculus

Symbol

Il1b^tm1Dch

Category

allele

Allele of gene

Il1b

Transgenic Constructs

None

Synonyms

• IL-1beta-
• IL-1BtmChaplin

Description

A PGK-neomycin resistance cassette was inserted into exon 4 that resulted in an in-frame termination codon upstream of the ICE cleavage site. The authors predict that the receptor-binding domain of the protein would not be translated. Northern blot analysis detected no transcripts in LPS-stimulated peritoneal exudate cells from homozygous mice. Pulse-chase labeling and immunoprecipitation studies of thioglycollate-elicited PEC demonstrated that no detectable protein was present in homozygous mice. ELIZA analysis of ATP-treated PEC also did not detect protein produced by this allele.

Additional Information

Literature

Transgenic Constructs

Genomic Variant Information

Variant Molecular Consequences

Phenotypes

Disease Associations