Version: 8.0.0Date: Tue Jan 28 2025
Allele/Variant
Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae
- Species
- Mus musculus
- Symbol
- Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae
- Category
- allele
- Allele of gene
- Col1a1
- Transgenic Constructs
- Synonyms
- Col1a1 2lox 4F2A
- Col1a1-2lox-4F2A
- Description
- RMCE on KH2 cells inserted a cassette that contains a loxP-flanked pgkNEOpolyA cassette, a third loxP site, the tetracycline responsive element (TRE or tetO), the single polycistronic expression cassette 4F2A, SV40 intron plus polyA signal, and a fourth loxP site into the 3'UTR. The 4F2A cassette contains the four mouse reprogramming genes Oct4 (Pou5f1; POU domain, class 5, transcription factor 1), Sox2 (SRY-box containing gene 2), Klf4 (Kruppel-like factor 4 (gut)), and c-Myc (Myc; myelocytomatosis oncogene) separated by three different viral 2A oligopeptides that mediate ribosomal skipping (P2A [from porcine teschovirus-1], T2A [from insect Thosea asigna virus], and E2A [equine rhinitis A virus], respectively).
- Additional Information
- Literature
Transgenic Constructs
This allele contains 1 transgenic construct- Symbol
- Expressed Components
- Knock-down Targets
- None
- Regulatory Regions
- None